Rate of Glucose Uptake by Yeast Cells Lab Report

Rate of Glucose Uptake by Yeast Cells - Lab score ExampleFrom the equation, it can be concluded that oneness breakwater of glucose reacts with both moles Cu2+ to give one mole of Cu2O. Thus, one can weigh the mass of Cu2O formed and relate it to the amount of glucose present in a solution. This method can be used to study the rate of glucose uptake by yeast cells.2. allow down the timer and add 1.00 mL of yeast suspension with a micropipette into one flask containing the glucose solution. Repeat this until 7 replicates argon obtained. Please note that this has to be done very fast (within 1 minute if possible). To the stay flasks, add 1.00 mL of distilled water into each, and label them as blank.4. After 30 minutes have elapsed, pullulate the contents of one flask labelled blank sample and one containing the yeast suspension into two expose beakers containing around 150 mL of boiling distilled water. Cool the mixtures to room temperature and transfer them into two separate 25 0-mL volumetric flasks. Add distilled water to the flasks to make a final volume of 250.00 mL. Shake the flasks well and let them stand until clear supernatants are obtained (the blank solution should not have a sediment if the investigate has been done properly). Label the one obtained from the blank sample as B60 and the other one as Y605. pipette 25.00 mL of supernatant from B60 and pour it in a 100 mL beaker. Add 25 mL of 0.2500 M Benedicts solution (it is in excess) to the beaker containing the supernatant from B60. Heat the resulting mixture to boiling until a red headlong is formed. Cool this mixture to room temperature. Repeat to get a replicate.6. Filter the mixture with a Gooch melting pot tared with filter paper using the set up shown in Figure 1. Wash the precipitate several(prenominal) times with cold distilled water.Figure 1. Set up for filtration apparatus7. Use a cloth (this would rid of fingerprints on the surface) to remove the crucible containing the precipita te from the filtration apparatus.8. Dry the sample to constant mass. Ensure that tongs are used to handle the crucible and that the sample is kept/cooled in a dessicator before weighing.9. Repeat travel 5-8 with Y60.10. Repeat steps 4-9 to get data at t =120,180,240,300,360,420 mins and label the samples accordingly. CalculationsAs per Equation 1, one mole of glucose reacts with two moles of Cu2+ to give one mole of Cu2O. In the above experiment, glucose is the limiting reagent and Cu2+ is in excess. whence the amount (mole) of Cu2O formed of is directly proportional to the amount of glucose in the solution.Molar mass of Cu2O = (63.55 x 2) + 16 = 143.10Molar mass of glucose, C6H12O6 = (12.01 x 6) + (1.01 x 12) + (16.00 x 6) = 180.18No. of moles of Cu2O formed =